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The remaining 23 mL of each culture was quickly filtered under vacuum (Air Cadet vacuum/pressure station, Thermo), immediately washed with cold saline solution (9.5 g L −1 NaCl at 1–2 °C) and quenched in cold methanol/water (1:1 v/v) at −30 °C as described by Smart et al
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2 The effectiveness of drug therapy is optimized by starting treatment early ( 2 years is 80 mg/kg in four divided doses for 5 days (maximum 3,200 mg daily)
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